Clostridium Medium

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium

培养基配方
Composition per liter:
Crotonic acid...........................................................6.0g
Pancreatic digest of casein.............................................1.0g
Yeast extract...........................................................1.0g
Sodium thioglycolate....................................................0.5g
NaOH...................................................................0.30g
(NH4)2HPO4 ............................................................0.15g
K2HPO4 ................................................................0.10g
NH4Cl .................................................................0.05g
CaCl2 ·2H2O...........................................................0.04g
MgCl2 ·6H2O..........................................................0.033g
(NH4)6Mo7O24 ·4H2O...................................................10.0mg
p-Aminobenzoic acid...................................................0.80mg
FeSO4 ·7H2O...........................................................0.6mg
MgSO4 ·7H2O...........................................................0.6mg
MnSO4 ·2H2O...........................................................0.4mg
Resazurin .............................................................1.0mg
Biotin ...............................................................0.04mg
K2CO3  solution.......................................................80.0mL
KHCO3  solution........................................................3.0mL
pH 6.8 ± 0.2 at 25°C
K2CO3 Solution:
Composition per 80.0mL:
K2CO3 ................................................................40.0g
Preparation of K2CO3 Solution:  Add K2CO3  to distilled/deion-
ized water and bring volume to 80.0mL. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
KHCO3 Solution:
Composition per 10.0mL:
KHCO3 .................................................................2.0g
Preparation of KHCO3 Solution:  Add KHCO3 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except K2CO3  solution
and KHCO3  solution, to distilled/deionized water and bring volume to
917.0mL. Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically and anaerobically add 80.0mL of
sterile K2CO3  solution and 3.0mL of sterile KHCO3  solution. Mix thor-
oughly. Aseptically and anaerobically distribute into sterile screw-
capped bottles under 100% N2 .
Use: For the cultivation of Clostridium halophilum and Clostridium
litorale.