Clostridium Medium (ATCC Medium 39)

[所属分类:培养基配方] [发布时间:2020-5-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium Medium
(ATCC Medium 39)

培养基配方
Composition per liter:
K2HPO4 ................................................................7.0g
γ-Aminobutyric acid...................................................5.0g
Yeast extract..........................................................3.0g
Agar...................................................................1.5g
KH2PO4 ................................................................1.3g
MgCl2 ·6H2O ..........................................................0.2g
CaCl2 ·2H2O .........................................................0.01g
FeCl3 ·6H2O..........................................................0.01g
Methylene Blue........................................................2.0mg
MnSO4 ................................................................1.0mg
Na2MoO4 ..............................................................1.0mg
Na2S·9H2O solution...................................................10.0mL
Na2S·9H2O Solution:
Composition per 20.0mL:
Na2S·9H2O.............................................................0.6g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 20.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Use freshly prepared solution.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Distribute anaerobically into sterile tubes.
Aseptically add 0.1mL of sterile 1.5% Na2S·9H2O solution to each
5.0mL of the medium. Cap with rubber stoppers.
Use:  For the cultivation and maintenance of a variety of Clostridium
species.