Clostridium kluyveri Agar

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium kluyveri Agar

培养基配方
Composition per 100.0mL:
Potassium acetate......................................................1.0g
Sodium thioglycolate.................................................50.0mg
K2HPO4 ..............................................................31.0mg
NH4·Cl .............................................................25.0mg
KH2PO4 ..............................................................23.0mg
MgSO4 ·7H2O.........................................................20.0mg
FeSO4 ·7H2O..........................................................2.0mg
MnSO4 ·H2O...........................................................2.0mg
CaCl2 ·2H2O..........................................................1.0mg
Na2MoO4 ·2H2O .......................................................0.2mg
Agar ...............................................................50.0μg
Resazurin ..........................................................50.0μg
p-Aminobenzoic acid.................................................20.0μg
Biotin .............................................................10.0μg
CaCO3 .............................................................variable
Ethanol..............................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except sodium thiogly-
colate, ethanol, and CaCO3 , to distilled/deionized water and bring vol-
ume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling.
Continue boiling for 5 min. Cool rapidly to 50°C. Add sodium thiogly-
colate and ethanol. Distribute into tubes containing a small amount of
CaCO3 . Autoclave for 15 min at 15 psi pressure–121°C. Store anaero-
bically.
Use:  For the cultivation and maintenance of Clostridium kluyveri.