Clostridium kluyveri Medium

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium kluyveri Medium

培养基配方
Composition per liter:
Part A...................................................................965.0mL
Part B....................................................................35.0mL
pH 7.0 ± 0.2 at 25°C
Part A:
Composition per 965.0mL:
Sodium acetate·3H2O.......................................................7.5g
(NH4)2SO4 ................................................................2.65g
Agar.......................................................................2.0g
Yeast extract..............................................................2.0g
Sodium thioglycolate.......................................................0.5g
p-Aminobenzoic acid.......................................................0.1mg
Biotin...................................................................5.0μg
Potassium phosphate
buffer (2M, pH 7.0) .....................................................10.0mL
Salt solution............................................................10.0mL
Preparation of Part A : Add components to distilled/deionized wa-
ter and bring volume to 965.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Salt Solution:
Composition per 100.0mL:
MgSO4 ·H2O...............................................................2.5g
CaCl2 ...................................................................0.15g
FeSO4 ·7H2O.............................................................0.15g
MnSO4 ·2H2O.............................................................0.02g
Na2MoO4 ·2H2O...........................................................0.02g
Preparation of Salt Solution : Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Part B:
Composition per liter:
K2CO3  (1M solution) ...................................................20.0mL
Ethanol (95% solution)..................................................15.0mL
Preparation of Part B: Prepare a 1M solution of K2CO3  and filter
sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically
combine 20.0mL of sterile K2CO3  solution and 15.0mL of sterile etha-
nol.
Preparation of Medium: Add 35.0mL of sterile Part B to 965.0mL
of sterile, cooled Part A. Adjust pH to 7.0 with HCl. Aseptically dis-
tribute into tubes under 97% N2 + 3% H2 . Cap with rubber stoppers.
Use: For the cultivation and maintenance of Clostridium kluyveri.