Clostridium hydroxybenzoicum Medium

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium hydroxybenzoicum Medium

培养基配方
Composition per 1055.0mL:
NaCl.........................................................................10.0g
Yeast extract................................................................10.0g
L -Arginine·HCl..............................................................2.1g
L -Lysine·HCl................................................................1.8g
Glycine......................................................................0.75g
NH 4 Cl ......................................................................0.5g
L -Cysteine·HCl .............................................................0.4g
Na2S·9H2O....................................................................0.4g
MgCl2 ·7H2O .................................................................0.1g
CaCl2 ·2H2O ...............................................................0.025g
Resazurin....................................................................1.0mg
Na2WO4 ·2H2O...............................................................0.05mg
Sodium/potassium phosphate buffer
(0.02M solution, pH 7.0)......................................................1.0L
Wolfe’s vitamin solution...................................................50.0mL
Wolfe’s mineral solution....................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl.............................................................10.0mg
p-Aminobenzoic acid..........................................................5.0mg
Lipoic acid..................................................................5.0mg
Nicotinic acid...............................................................5.0mg
Riboflavin...................................................................5.0mg
Thiamine·HCl................................................................5.0mg
Calcium  DL -pantothenate....................................................5.0mg
Biotin.......................................................................2.0mg
Folic acid ..................................................................2.0mg
Vitamin B 12 ................................................................0.1mg
Preparation of Wolfe’s Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Wolfe’s Mineral Solution:
Composition per liter:
MgSO4 ·7H2O..................................................................3.0g
Nitrilotriacetic acid.........................................................1.5g
NaCl..........................................................................1.0g
MnSO4 ·2H2O..................................................................0.5g
CoCl2 ·6H2O..................................................................0.1g
ZnSO4 ·7H2O..................................................................0.1g
CaCl2 ·2H2O .................................................................0.1g
FeSO4 ·7H2O..................................................................0.1g
NiCl2 ·6H2O................................................................0.025g
KAl(SO4)2 ·12H2O............................................................0.02g
CuSO4 ·5H2O.................................................................0.01g
H3BO3 .......................................................................0.01g
Na2MoO4 ·2H2O...............................................................0.01g
Na2SeO3 ·5H2O...............................................................0.3mg
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L. Adjust pH to 6.8.
Preparation of Medium: Prepare and dispense medium under
100% N2 . Combine components. Mix thoroughly. Sparge with 100%
N2 . Anaerobically distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation of Clostridium hydroxybenzoicum.