Clostridium HiVeg Broth Base with Lactate

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium HiVeg Broth Base with Lactate

培养基配方
Composition per liter:
Plant hydrolysate........................................................15.0g
Plant extract No.1 ......................................................10.0g
Sodium acetate............................................................5.0g
Yeast extract.............................................................5.0g
L -Cysteine...............................................................0.5g
Lactate solution........................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium, without lactate, is available as a premixed
powder from HiMedia.
Lactate Solution:
Composition per10.0mL:
Sodium lactate...........................................................5.0g
Preparation of Lactate Solution: Add sodium lactate to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Heat if
necessary to completely dissolve.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the identification of spores of Clostridium tyrobutyricum
which is usually responsible for “late blowing” in cheese.