Clostridium formicoaceticum Agar

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium formicoaceticum Agar

培养基配方
Composition per liter:
Agar ......................................................................15.0g
K2HPO4 ....................................................................10.0g
Yeast extract...............................................................5.0g
Sodium thioglycolate.......................................................0.75g
Pyridoxine·HCl............................................................1.0mg
Resazurin .................................................................1.0mg
Fructose solution.........................................................50.0mL
NaHCO3  solution .........................................................30.0mL
Trace elements solution SL-4 .............................................10.0mL
pH 8.0 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA.......................................................................0.5g
FeSO4 ·7H2O...............................................................0.2g
Trace elements solution SL-6 ...........................................100.0mL
Trace Elements Solutions SL-6:
Composition per liter:
MnCl2 ·4H2O..............................................................0.5g
H3BO3 ....................................................................0.3g
CoCl2 ·6H2O .............................................................0.2g
ZnSO4 ·7H2O..............................................................0.1g
Na2MoO4 ·2H2O ..........................................................0.03g
NiCl2 ·6H2O.............................................................0.02g
CuCl2 ·2H2O ............................................................0.01g
Preparation of Trace Elements Solution SL-6:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Fructose Solution:
Composition per 50.0mL:
Fructose...................................................................5.0g
Preparation of Fructose Solution : Add fructose to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
under 100% N2 gas for 3 min. Filter sterilize. Store under N2 gas.
NaHCO3 Solution:
Composition per 30.0mL:
NaHCO3 ...................................................................10.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 30.0mL. Mix thoroughly. Sparge
with 100% CO2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except fructose solu-
tion and NaHCO3  solution, to distilled/deionized water and bring vol-
ume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling.
Cool to 50°C while sparging with 100% CO2 . Autoclave for 15 min at
15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL of
sterile fructose solution and 30.0mL of sterile NaHCO3  solution. Mix
thoroughly. Adjust pH to 8.0. Aseptically and anaerobically pour into
sterile Petri dishes or distribute into sterile screw-capped bottles under
100% CO2 .
Use: For the cultivation and maintenance of Clostridium formicoace-
ticum.