Clostridium estertheticum Medium

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium estertheticum Medium

培养基配方
Tryptose................................................................10.0g
Beef extract............................................................10.0g
Glucose..................................................................5.0g
NaCl.....................................................................5.0g
Yeast extract............................................................3.0g
Sodium acetate...........................................................3.0g
Soluble starch...........................................................1.0g
L -Cysteine·HCl·H2O....................................................0.5g
Agar.....................................................................0.5g
Glucose solution ......................................................90.0mL
NaHCO3  solution.......................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Glucose Solution:
Composition per 90.0mL:
D -Glucose..............................................................10.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 90.0mL. Mix thoroughly. Sparge with
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
NaHCO3 Solution:
Composition per 10.0mL:
NaHCO3 ................................................................2.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge
with 80% N2 + 20% CO2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Prepare and dispense medium under 80%
N2 + 20% CO2 gas. Add components, except glucose solution and
NaHCO3 solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust pH to 6.8. Sparge with 80% N2 +
20% CO2 gas. Autoclave for 15 min at 15 psi pressure–121°C. Asepti-
cally and anaerobically add 90.0mL of sterile glucose solution and
10.0mL of sterile NaHCO3 solution. Mix thoroughly. Aseptically and
anaerobically distribute into sterile tubes or bottles.
Use: For the cultivation of Clostridium estertheticum.