​Clostridium formicoaceticum Broth

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium formicoaceticum Broth

培养基配方
Composition per liter:
K2HPO4 ...................................................................10.0g
Yeast extract..............................................................5.0g
Sodium thioglycolate......................................................0.75g
Pyridoxine·HCl...........................................................1.0mg
Resazurin.................................................................1.0mg
Fructose solution........................................................50.0mL
NaHCO3  solution.........................................................30.0mL
Trace elements solution SL-4.............................................10.0mL
pH 8.0 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA.......................................................................0.5g
FeSO4 ·7H2O...............................................................0.2g
Trace elements solution SL-6............................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O ..............................................................0.5g
H3BO3 .....................................................................0.3g
CoCl2 ·6H2O...............................................................0.2g
ZnSO4 ·7H2O...............................................................0.1g
Na2MoO4 ·2H2O............................................................0.03g
NiCl2 ·6H2O..............................................................0.02g
CuCl2 ·2H2O..............................................................0.01g
Preparation of Trace Elements Solution SL-6:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Fructose Solution:
Composition per 50.0mL:
Fructose...................................................................5.0g
Preparation of Fructose Solution : Add fructose to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
under 100% N2 gas for 3 min. Filter sterilize. Store under N2 gas.
NaHCO3 Solution:
Composition per 30.0mL:
NaHCO3 ..................................................................10.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 30.0mL. Mix thoroughly. Sparge
with 100% CO2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except fructose solu-
tion and NaHCO3  solution, to distilled/deionized water and bring vol-
ume to 920.0mL. Mix thoroughly. Sparge with 100% CO2 . Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add
50.0mL of sterile fructose solution and 30.0mL of sterile NaHCO3  so-
lution. Mix thoroughly. Adjust pH to 8.0. Aseptically and anaerobical-
ly distribute into sterile screw-capped bottles under 100% CO2 .
Use: For the cultivation of Clostridium formicoaceticum.