Clostridium difficile HiVeg Agar Base

[所属分类:培养基配方] [发布时间:2020-5-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium difficile HiVeg Agar Base

培养基配方

Composition per liter:
Plant peptone No.3........................................................40.0g
Agar .....................................................................15.0g
Fructose...................................................................6.0g
Na2HPO4 ...................................................................5.0g
NaCl.......................................................................2.0g
KH2PO4 ....................................................................1.0g
MgSO4 .....................................................................0.1g
Horse blood, defibrinated ...............................................70.0mL
Clostridium difficile selective supplement...............................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement, is
available as a premixed powder from HiMedia.
Clostridium difficile Selective Supplement:
Composition per 10.0mL:
D- Cycloserine.........................................................500.0mg
Cefoxitin...............................................................16.0mg
Preparation of Clostridium difficile Selective Supplement:
Add components to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except blood and se-
lective supplement, to distilled/deionized water and bring volume to
920.0mL. Mix thoroughly. Gently heat to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Add 10.0mL of ster-
ile Clostridium difficile selective supplement and 70.0mL of sterile, de-
fibrinated horse blood. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective isolation and cultivation of Clostridium difficile
from fecal specimens.