Clostridium difficile Agar

[所属分类:培养基配方] [发布时间:2020-5-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium difficile Agar

培养基配方
Composition per liter:
Clostridum difficile agar base..............................................920.0mL
Horse blood, defibrinated ...................................................70.0mL
Clostridium difficile
selective supplement ........................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Clostridum difficile Agar Base:
Composition per 920.0mL:
Proteose peptone...............................................................40.0g
Agar...........................................................................15.0g
Fructose........................................................................6.0g
Na2HPO4 ........................................................................5.0g
NaCl............................................................................2.0g
KH2PO4 .........................................................................1.0g
MgSO4 ·7H2O....................................................................0.1g
Preparation of Clostridium difficile Agar Base: Add compo-
nents to distilled/deionized water and bring volume to 920.0mL. Mix
thoroughly. Gently heat to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Clostridium difficile Selective Supplement:
Composition per 10.0mL:
D- Cycloserine..............................................................500.0mg
Cefoxitin ...................................................................16.0mg
Preparation of Clostridium difficile Selective Supplement:
Add components to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add 10.0mL of sterile Clostridium dif-
ficile selective supplement and 70.0mL of sterile, defibrinated horse
blood to 920.0mL of cooled, sterile Clostridium difficile agar base. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Clostridium difficile
from clinical and nonclinical specimens.