Clostridium difficile Agar (Cycloserine Cefoxitin Fructose Agar) (CCFA)

[所属分类:培养基配方] [发布时间:2020-5-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium difficile Agar
(Cycloserine Cefoxitin Fructose Agar)
(CCFA)

培养基配方
Composition per liter:
Peptic digest of animal tissue............................................32.0g
Agar .....................................................................20.0g
Fructose...................................................................6.0g
Na2HPO4 ...................................................................5.0g
NaCl.......................................................................2.0g
KH2PO4 ....................................................................1.0g
Cycloserine ..............................................................0.25g
MgSO4 .....................................................................0.1g
Neutral Red...............................................................0.03g
Cefoxitin solution ......................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Cefoxitin Solution:
Composition per 10.0mL:
Cefoxitin ..............................................................16.0mg
Preparation of Cefoxitin Solution: Add cefoxitin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly. Gently heat to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL of sterile cefoxitin solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the selective isolation and cultivation of Clostridium difficile
from clinical and nonclinical specimens.