Clostridium CK Medium (DSMZ Medium 869)

[所属分类:培养基配方] [发布时间:2020-5-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium CK Medium
(DSMZ Medium 869)

培养基配方
Composition per liter:
Pancreatic digest of casein...................................................3.2g
NaCl..........................................................................0.9g
Papaic digest of soybean meal.................................................0.6g
K2HPO4 .......................................................................0.5g
Glucose.......................................................................0.5g
Resazurin....................................................................0.5mg
Glucose solution.............................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose........................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with
100% N2 . Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 5.5. Sparge with 100% N2 . Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobi-
cally add 10.0mL sterile glucose solution. Aseptically and anaerobical-
ly distribute into tubes or flasks.
Use: For the cultivation of Clostridium akagii, Clostridium acidisoli,
and Clostridium uliginosum.