Clostridium chauvoei Blood Agar

[所属分类:培养基配方] [发布时间:2020-5-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium chauvoei Blood Agar

培养基配方
Composition per 100.0mL:
Liver extract....................................................................3.0g
Agar ............................................................................1.6g
Glucose .........................................................................1.0g
VL broth base..................................................................94.0mL
Sheep blood, defibrinated.......................................................5.0mL
pH 7.2–7.4 at 25°C
VL Broth Base:
Composition per liter:
Pancreatic digest of casein.....................................................10.0g
NaCl.............................................................................5.0g
Yeast extract....................................................................5.0g
Meat extract ....................................................................2.0g
Agar ............................................................................0.6g
L -Cysteine·HCl·H2O ...........................................................0.4g
Preparation of VL Broth Base: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
until dissolved. Adjust pH to 7.2–7.4.
Preparation of Medium: Add liver extract, glucose, and agar to
94.0mL of VL broth base. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Clostridium chauvoei.