Brilliant Green HiVeg Agar

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green HiVeg Agar

培养基配方
Composition per liter:
Agar ......................................................10.15g
Plant peptone...............................................8.25g
Lactose......................................................1.9g
Basic Fuchsin.............................................776.0mg
Erioglaucine..............................................649.0mg
FeCl3 ....................................................295.0mg
Na2SO3 ...................................................205.0mg
KH2PO4 ....................................................15.3mg
Synthetic detergent No.II..................................2.95mg
Brilliant Green ..........................................29.5μg
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the selective isolation of Salmonella other than Salmonella
typhi from feces and other specimens, and food and dairy products.
Salmonella other than Salmonella typhi appear as red/pink/white col-
onies surrounded by a zone of red in the agar, indicating nonlactose/
sucrose fermentation. Proteus or Pseudomonas species may appear as
small red colonies. Lactose- or sucrose-fermenting bacteria appear as
yellow-green colonies surrounded by a zone of yellow-green in the
agar.