Brilliant Green HiVeg Agar Base with Sulfa Supplement

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green HiVeg Agar Base
with Sulfa Supplement

培养基配方
Composition per liter:
Agar......................................................20.0g
Plant peptone No.3........................................10.0g
Lactose...................................................10.0g
Sucrose...................................................10.0g
NaCl.......................................................5.0g
Yeast extract..............................................3.0g
Phenol Red................................................0.08g
Brilliant Green.........................................125.0mg
Sulfa supplement solution................................10.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium, without sulfa supplement, is available as a pre-
mixed powder from HiMedia.
Sulfa Supplement Solution:
Composition per 10.0mL:
Sodium sulfacetamide......................................1.0g
Sodium mandelate ........................................0.25g
Preparation of Sulfa Supplement Solution:  Add components to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except sulfa supple-
ment solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile sulfa supplement solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the selective isolation of Salmonella other than Salmonella
typhi from feces and other specimens.