Brilliant Green Broth (m-Brilliant Green Broth)

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Broth
(m-Brilliant Green Broth)

培养基配方
Composition per liter:
Proteose peptone No.3.......................................20.0g
Lactose.....................................................20.0g
Sucrose.....................................................20.0g
NaCl........................................................10.0g
Yeast extract................................................6.0g
Phenol Red..................................................0.16g
Brilliant Green ...........................................0.025g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat with fre-
quent mixing. Boil for 1 min. Cool to 25°C. Add 2.0mL to each sterile
absorbent filter used.
Use: For the selective isolation and differentiation of Salmonella from
polluted water by the membrane filter method.