Brain Heart Infusion with Rabbit Serum and Yeast Extract

[所属分类:培养基配方] [发布时间:2020-1-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with
Rabbit Serum and Yeast Extract

培养基配方
Composition per liter:
Pancreatic digest of casein.................................16.0g
Brain heart, solids from infusion ...........................8.0g
Peptic digest of animal tissue...............................5.0g
NaCl.........................................................5.0g
Glucose .....................................................2.0g
Na2HPO4 .....................................................2.5g
Rabbit serum, heat inactivated............................200.0mL
Fresh yeast extract (25% solution)........................100.0mL
pH 7.2 ± 0.2 at 25°C
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free.................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 800.0mL. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add
200.0mL of sterile, heat-inactivated rabbit serum. Distribute aseptical-
ly into tubes or flasks.
Use:  For the cultivation and maintenance of Mycoplasma equirhinis.