Brain Heart Infusion with Serum and Glucose (BHI with Serum and Glucose)

[所属分类:培养基配方] [发布时间:2020-1-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Serum and Glucose
(BHI with Serum and Glucose)

培养基配方
Composition per liter:
Pancreatic digest of gelatin..................................14.5g
Brain heart, solids from infusion .............................6.0g
Peptic digest of animal tissue.................................6.0g
NaCl...........................................................5.0g
Glucose .......................................................3.0g
Na2HPO4 .......................................................2.5g
Glucose .......................................................2.0g
Bovine serum, heat inactivated..............................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium anaerobi-
cally under 80% N2 + 10% CO2 + 10% H2 . Add components, except
heat-inactivated fetal bovine serum, to distilled/deionized water and
bring volume to 900.0mL. Mix thoroughly. Sparge with 80% N2 + 10%
CO2 + 10% H2 gas mixture. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile fetal bovine serum. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes under an atmosphere of 80%
N2 + 10% CO2 + 10% H2 .
Use: For the cultivation of Actinomyces hordeovulneris, Actinomyces
hyovaginalis, Serpulina hyodysenteriae, Serpulina pilosicoli, and
Treponema phagedenis.