Brain Heart Infusion with Rabbit Serum

[所属分类:培养基配方] [发布时间:2020-1-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Rabbit Serum

培养基配方
Composition per liter:
Yeast extract.............................................20.0g
Pancreatic digest of casein...............................16.0g
Brain heart, solids from infusion..........................8.0g
Peptic digest of animal tissue ............................5.0g
NaCl.......................................................5.0g
Na2HPO4 ...................................................2.5g
Glucose....................................................2.0g
Rabbit serum, heat inactivated..........................50.0mL
Nicotinamide adenine
dinucleotide solution...................................10.0mL
pH 7.2 ± 0.2 at 25°C
Nicotinamide Adenine Dinucleotide Solution:
Composition per 10.0mL:
Nicotinamide adenine dinucleotide .......................0.1g
Preparation of Nicotinamide Adenine Dinucleotide Solution:
Add 0.1g of nicotinamide adenine dinucleotide to distilled/deinonized
water and bring volume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except nicotinamide
adenine dinucleotide solution and rabbit serum, to distilled/deionized
water and bring volume to 940.0mL. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of a 0.1%
filter-sterilized solution of nicotinamide adenine dinucleotide. Asepti-
cally add 50.0mL of heat-inactivated rabbit serum. Distribute asepti-
cally into tubes or flasks.
Use:  For the cultivation and maintenance of Actinobacillus species.