Blood Agar No. 2

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar No.2

培养基配方
Composition per liter:
Proteose peptone............................................15.0g
Agar........................................................12.0g
NaCl.........................................................5.0g
Yeast extract................................................5.0g
Liver digest.................................................2.5g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat with frequent
agitation and boil for 1 min to completely dissolve. Autoclave for 15
min at 15 psi pressure–121°C. Cool the basal medium to 45°–50°C.
Aseptically add sterile, defibrinated blood to a final concentration of
7%. Pour into sterile Petri dishes.
Use:  For the isolation, cultivation, and detection of hemolytic activity of
streptococci, pneumococci, and other particularly fastidious microorgan-
isms.