Blood Agar with Low pH

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar with Low pH

培养基配方
Composition per liter:
Beef heart, solids from infusion...........................500.0g
Agar........................................................15.0g
Tryptose....................................................10.0g
NaCl.........................................................5.0g
Sheep blood, defibrinated..................................50.0mL
pH 6. 8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Heat with frequent agitation and boil for 1 min to completely dis-
solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix
thoroughly and pour into sterile Petri dishes.
Use:  For the isolation and growth of a wide variety of microorgan-
isms. For the detection of the hemolytic reactions of streptococci and
other fastidious microorganisms. The slightly acid pH of this medium
enhances distinct hemolytic reactions.