Blood Agar, Diphasic Base Medium

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar, Diphasic Base Medium

培养基配方
Composition per 750.0mL:
Beef.........................................................25.0g
Agar ........................................................10.0g
Neopeptone...................................................10.0g
NaCl..........................................................2.5g
pH 7.2–7.4 at 25°C
Preparation of Medium: Trim beef to remove fat. Add 25.0g of
lean beef to 250.0mL of distilled/deionized water. Gently heat and
bring to boiling. Boil for 2–3 min. Filter through Whatman #2 filter pa-
per. Add agar, neopeptone, and NaCl to filtrate. Bring volume to
750.0mL with distilled/deionized water. Mix thoroughly. Adjust pH to
7.2–7.4. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or distribute into ster-
ile tubes.
Use: For the cultivation of Trypanosoma species.