Blood Agar Base No. 2 (BAM M22)

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base No.2
(BAM M22)

培养基配方
Composition per 1004.0mL:
Agar .......................................................12.0g
Proteose peptone............................................15.0g
NaCl.........................................................5.0g
Yeast extract................................................5.0g
Liver digest.................................................2.5g
Horse blood, defibrinated .................................50.0mL
FBP solution.................................................4.0mL
pH 7.4 ± 0.2 at 25°C
FBP Solution:
Composition per 30.0mL:
FeSO4 .........................................................0.25g
NaHSO3 ........................................................0.25g
Sodium pyruvate.................................................0.25g
Preparation of FBP Solution: Add components to distilled/deion-
ized water and bring volume to 30.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except horse blood and
FBP solution, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 48°C. Aseptically add
50.0mL of sterile horse blood. Mix thoroughly. Aseptically add 4.0mL
sterile FBP solution. Mix thoroughly. Pour into sterile Petri dishes in
20.0mL volumes.
Use: For the cultivation of Brucella spp. and other fastidious bacteria.