Blood Agar Base No. 2, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base No.2, HiVeg with Blood

培养基配方
Composition per liter:
Agar........................................................15.0g
Plant peptone No.3..........................................15.0g
NaCl.........................................................5.0g
Yeast extract................................................5.0g
Plant extract No.2...........................................2.5g
Horse blood, defibrinated .................................70.0mL
Selective supplement..........................................2 vials
pH 7.4 ± 0.2 at 25°C
Source: This medium without blood or supplement is available as a
premixed powder from HiMedia.
Preparation of Medium: Add components, except horse blood and
selective supplement, to distilled/deionized water and bring volume to
930.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 70.0mL of sterile horse blood. Mix thoroughly. Aseptically add 2
vials of rehydrated selective supplement. For Brucella spp. use Brucel-
la selective supplement. For Campylobacter spp. use Campylobacter
supplement-I (Blaser-Wang), or Campylobacter Supplement II (But-
zler), or Campylobacter Supplement III (Skirrow), or Campylobacter
Growth Supplement. For streptococci use Strepto supplement. Mix
thoroughly. Pour into sterile Petri dishes in 20.0mL volumes.
Use:  For the cultivation of Brucella spp., Campylobacter spp., Strep-
tococcus spp., and other fastidious bacteria.