Blood Agar Base with Special Peptone

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base with Special Peptone

培养基配方
Composition per liter:
Agar .....................................................15.0g
Beef extract..............................................10.0g
Special peptone...........................................10.0g
NaCl.......................................................5.0g
Sheep blood, defibrinated................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: Special peptone (L72) is available from Oxoid Unipath.
Preparation of Medium:  Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Heat with frequent agitation and boil for 1 min to completely dis-
solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix
thoroughly and pour into sterile Petri dishes.
Use:  For the isolation, cultivation, and detection of hemolytic activity
of streptococci and other fastidious microorganisms.