Blood Agar Base with 3.5% Sodium Chloride

[所属分类:培养基配方] [发布时间:2019-12-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Agar Base with 3.5% Sodium Chloride

培养基配方
Composition per liter:
Beef heart, infusion from................................500.0g
NaCl......................................................40.0g
Agar .....................................................15.0g
Tryptose .................................................10.0g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat with frequent
agitation and boil for 1 min to completely dissolve. Autoclave for 15
min at 15 psi pressure–121°C. Cool the basal medium to 45°–50°C.
Aseptically add sterile, defibrinated blood to a final concentration of
5%. Mix thoroughly and pour into sterile Petri dishes.
Use: For the cultivation of Vibrio costicola.