Biphenyl Agar (DSMZ Medium 457d)

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Biphenyl Agar
(DSMZ Medium 457d)

培养基配方
Composition per liter:
Agar...........................................................15.0g
Na2HPO4 .......................................................2.44g
KH2PO4 ........................................................1.52g
(NH4)2SO4 ......................................................0.5g
Biphenyl.......................................................0.25g
MgSO4 ·7H2O....................................................0.2g
CaCl2 ·2H2O...................................................0.05g
Trace elements solution SL-4 .................................10.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................0.5g
FeSO4 ·7H2O....................................................0.2g
Trace elements solution SL-6 ................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H3BO3 ..........................................................0.3g
CoCl2 ·6H2O ...................................................0.2g
ZnSO4 ·7H2O....................................................0.1g
MnCl2 ·4H2O...................................................0.03g
Na2MoO4 ·H2O .................................................0.03g
NiCl2 ·6H2O...................................................0.02g
CuCl2 ·2H2O...................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Biphenyl Solution:
Composition per liter:
Biphenyl.......................................................10.0g
Preparation of Biphenyl Solution: Add biphenyl to 1.0L ethanol.
Mix thoroughly. Filter sterilize using a cellulose filter membrane.
Preparation of Medium: Add components, except biphenyl solu-
tion, to 1.0L distilled/deionized water. Adjust pH to 6.9. Heat and gently
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Add an aliquot of the biphenyl solution to the lid of a sterile Petri
dish so that the final concentration will be approximately 0.25g/L bi-
phenyl, and let the ethanol evaporate so that the crystals of biphenyl
coat the lid of the Petri dish. Aseptically add sterile agar medium to the
crystal-layered Petri dish.
Use: For the cultivation of biphenyl-utilizing bacteria.