Biphasic Medium for Neisseria

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Biphasic Medium for Neisseria

培养基配方
Composition per liter:
Glucose starch agar............................................1.0L
Glucose starch broth...........................................1.0L
pH 7.3 ± 0.2 at 25°C
Glucose Starch Agar:
Composition per liter:
Agar..........................................................20.0g
Gelatin.......................................................20.0g
Proteose peptone No. 3........................................15.0g
Soluble starch................................................10.0g
NaCl...........................................................5.0g
Glucose........................................................2.0g
Na2HPO4 .......................................................3.0g
Preparation of Glucose Starch Agar: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C.
Glucose Starch Broth:
Composition per liter:
Gelatin.........................................................20.0g
Proteose peptone No.3...........................................15.0g
Soluble starch..................................................10.0g
NaCl.............................................................5.0g
Glucose..........................................................2.0g
Na2HPO4 .........................................................3.0g
Preparation of Glucose Starch Broth: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C.
Preparation of Medium: Aseptically distibute glucose starch agar
into flasks in 100–125mL volumes. Allow agar to solidify. Overlay
agar with 25.0mL of sterile glucose starch broth.
Use:  For selective isolation and cultivation of Neisseria species.