Bird Seed Agar (Guizotia abyssinica Creatinine Agar) (Niger Seed Agar)/(Staib Agar)

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bird Seed Agar
(Guizotia abyssinica Creatinine Agar)
(Niger Seed Agar)/(Staib Agar)

培养基配方
Composition per liter:
Agar ...........................................................15.0g
Glucose ........................................................15.0g
Creatinine.......................................................5.0g
KH2PO4 ..........................................................3.0g
Biphenyl.........................................................1.0g
Chloramphenicol..................................................0.5g
Guizotia abyssinica seed
(niger seed) extract........................................1000.0mL
pH 6.7 ± 0.2 at 25°C
Preparation of Medium: Prepare seed extract by grinding 50.0g of
Guizotia abyssinica seed in 1.0L of distilled/deionized water. Boil for
30 min. Filter through cheesecloth and filter paper. Add remaining
components to seed filtrate. Mix thoroughly and heat with frequent ag-
itation until boiling. Distribute into flasks or tubes. Autoclave for 25
min at 15 psi pressure–110°C.
Use:  For the selective isolation and differentiation of Cryptococcus
neoformans from other Cryptococcus species and other yeasts.