Biotin Assay Medium

[所属分类:培养基配方] [发布时间:2019-12-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Biotin Assay Medium

培养基配方
Composition per liter:
Glucose ....................................................40.0g
Sodium acetate..............................................20.0g
Vitamin assay casamino acids................................12.0g
K2HPO4 ...................................................1.0g
KH2PO4 ...................................................1.0g
MgSO4 ·7H2O..............................................0.4g
DL- Tryptophane..............................................0.2g
L- Cystine ..................................................0.2g
Adenine sulfate.............................................0.02g
FeSO4 .....................................................0.02g
Guanine·HCl................................................0.02g
MgSO4 ·7H2O.............................................0.02g
NaCl........................................................0.02g
Uracil .....................................................0.02g
Calcium pantothenate .......................................2.0mg
Niacin......................................................2.0mg
Pyridoxine·HCl.............................................2.0mg
Riboflavin..................................................2.0mg
Thiamine·HCl ..............................................2.0mg
p-Aminobenzoic acid.........................................0.2mg
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL
volumes. Add standard solution or test solutions to each tube. Adjust
the volume of each tube to 10.0mL with distilled/deionized water. Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For use in the microbiological assay of biotin using Lactobacillus
plantarum as the test microorganism.