Bile Esculin HiVeg Agar with Kanamycin

[所属分类:培养基配方] [发布时间:2019-12-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin HiVeg Agar with Kanamycin

培养基配方
Composition per liter:
Plant peptone no.2...........................................17.0g
Agar ........................................................15.0g
Plant extract ................................................6.0g
Synthetic detergent...........................................5.0g
Esculin ......................................................1.0g
Ferric citrate................................................0.5g
Kanamycin.....................................................0.1g
Fe4(P2O7)3 ·H2O.............................................0.01g
Vitamin K 1 .................................................0.01g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation : Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly and heat with frequent agitation until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes or
leave in tubes. Cool tubes in a slanted position.
Use:  For the selective isolation and/or presumptive identification of
bacteria of the Bacteroides fragilis group from specimens containing
mixed flora. Examine colonies with a long-wavelength UV light. Pig-
mented colonies of the Bacteroides group will fluoresce red-orange.
Growth on this medium with blackening of the medium is presumptive
for Bacteroides fragilis.