Bile Esculin HiVeg Agar Base with Esculin

[所属分类:培养基配方] [发布时间:2019-12-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin HiVeg Agar Base with Esculin

培养基配方
Composition per liter:
Plant peptone ...............................................22.0g
Agar.........................................................15.0g
Plant hydrolysate ...........................................15.0g
Plant extract.................................................6.0g
Synthetic detergent No. II....................................5.0g
Ferric citrate................................................0.5g
Esculin solution ............................................4.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium, without esculin, is available as a premixed
powder from HiMedia.
Esculin Solution:
Composition per 4.0mL:
Esculin.......................................................1.0g
Esculin Solution: Add esculin to distilled/deionized water and bring
volume to 4.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except esculin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 4.0mL of sterile escu-
lin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile test tubes.
Use: For the isolation and presumptive identification of group D strep-
tococci.