Bile Oxalate Sorbose Broth (BOS Broth)

[所属分类:培养基配方] [发布时间:2019-12-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Oxalate Sorbose Broth
(BOS Broth)

培养基配方
Composition per liter:
Na2HPO4 ....................................................9.14g
Sodium oxalate ..............................................5.0g
Bile salts...................................................2.0g
NaCl.........................................................1.0g
CaCl2 ·2H2O................................................0.01g
MgSO4 ·7H2O................................................0.01g
Asparagine solution ......................................100.0mL
Methionine solution.......................................100.0mL
Sorbose solution..........................................100.0mL
Yeast extract solution.....................................10.0mL
Sodium pyruvate solution...................................10.0mL
Metanil Yellow solution....................................10.0mL
Sodium nitrofurantoin solution.............................10.0mL
Irgasan ® solution .........................................1.0mL
pH 7.6 ± 0.2 at 25°C
Asparagine Solution:
Composition per 100.0mL:
Asparagine..................................................1.0g
Preparation of Asparagine Solution: Add asparagine to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Methionine Solution:
Composition per 100.0mL:
Methionine..................................................1.0g
Preparation of Methionine Solution: Add methionine to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Sorbose Solution:
Composition per 100.0mL:
Sorbose ......................................................10.0g
Preparation of Sorbose Solution: Add sorbose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract...............................................0.025g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate.............................................0.05g
Preparation of Sodium Pyruvate Solution: Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Metanil Yellow Solution:
Composition per 10.0mL:
Metanil Yellow ............................................0.025g
Preparation of Metanil Yellow Solution:  Add Metanil Yellow to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Sodium Nitrofurantoin Solution:
Composition per 10.0mL:
Sodium nitrofurantoin.......................................0.01g
Preparation of Sodium Nitrofurantoin Solution: Add sodium
nitrofurantoin to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Irgasan ® Solution:
Composition per 10.0mL:
Irgasan.....................................................0.04g
Ethanol (95% solution)......................................10.0mL
Preparation of Irgasan Solution: Add Irgasan to 10.0mL of etha-
nol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except asparagine solu-
tion, methionine solution, sorbose solution, yeast extract solution, sodi-
um pyruvate solution, Metanil Yellow solution, sodium nitrofurantoin
solution, and Irgasan solution, to distilled/deionized water and bring vol-
ume to 659.0mL. Mix thoroughly. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster-
ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of
sterile yeast extract solution, 10.0mL of sterile sodium pyruvate solution,
10.0mL of sterile Metanil Yellow solution, 10.0mL of sterile sodium ni-
trofurantoin solution, and 1.0mL of sterile Irgasan solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Yersinia enterocolitica from
foods.