Bile Esculin Agar with Kanamycin

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Agar with Kanamycin

培养基配方
Composition per liter:
Oxgall........................................................20.0g
Agar .........................................................15.0g
Beef extract...................................................3.0g
Esculin .......................................................1.0g
Ferric citrate.................................................0.5g
Hemin........................................................10.0mg
Vitamin K1 ..................................................10.0mg
Horse serum..................................................50.0mL
Kanamycin solution...........................................10.0mL
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Kanamycin Solution:
Composition per 10.0mL:
Kanamycin.....................................................1.0g
Preparation of Kanamycin Solution: Add kanamycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 50.0mL of 5% filter-sterilized horse
serum and 10.0mL of sterile kanamycin solution. Distribute into test
tubes or flasks. Cool tubes in a slanted position.
Use:  For the selective isolation and/or presumptive identification of
bacteria of the Bacteroides fragilis group from specimens containing
mixed flora. Examine colonies with a long-wavelength UV light. Pig-
mented colonies of the Bacteroides group will fluoresce red-orange.
Growth on this medium with blackening of the medium is presumptive
for Bacteroides fragilis.