Bile Esculin Agar, HiVeg

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Agar, HiVeg

培养基配方
Composition per liter:
Plant peptone ..............................................25.0g
Agar........................................................15.0g
Plant hydrolysate ..........................................15.0g
Plant extract................................................6.0g
Synthetic detergent No. II...................................2.0g
Esculin .....................................................1.0g
Ferric citrate...............................................0.5g
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Distribute into sterile Petri dishes or test tubes. Cool tubes in a slanted
position.
Use: For the isolation and presumptive identification of group D strep-
tococci.