Bile Esculin Azide Agar

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Azide Agar

培养基配方
Composition per liter:
Pancreatic digest of casein.....................................17.0g
Agar ...........................................................15.0g
Oxgall..........................................................10.0g
NaCl.............................................................5.0g
Yeast extract....................................................5.0g
Proteose peptone No.3............................................3.0g
Esculin .........................................................1.0g
Ferric ammonium citrate..........................................0.5g
NaN 3 ..........................................................0.15g
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation : Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly and heat with frequent agitation until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes or
leave in tubes. Cool tubes in a slanted position.
Use: For the isolation and presumptive identification of group D strep-
tococci.