Bile Esculin Agar

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Agar

培养基配方
Composition per liter:
Esculin ......................................................1.0g
Bile esculin agar base .......................................1.0L
pH 6.6 ± 0.2 at 25°C
Bile Esculin Agar Base:
Composition per liter:
Oxgall........................................................40.0g
Agar .........................................................15.0g
Peptone .......................................................5.0g
Beef extract...................................................3.0g
Ferric citrate.................................................0.5g
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Bile Esculin Agar Base: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add desired amount of esculin—typical-
ly 1.0g—to bile esculin agar base. Mix thoroughly and heat with fre-
quent agitation until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Distribute into sterile Petri dishes or test
tubes. Cool tubes in a slanted position.
Use: For the isolation and presumptive identification of group D strep-
tococci.