Bile Esculin Agar

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Agar

培养基配方
Composition per liter:
Oxgall.....................................................20.0g
Agar ......................................................15.0g
Pancreatic digest of gelatin................................5.0g
Beef extract................................................3.0g
Esculin ....................................................1.0g
Ferric citrate..............................................0.5g
Horse serum...............................................50.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 950.0L. Mix thoroughly
and heat with frequent agitation until boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of
filter sterilized horse serum. Distribute into sterile Petri dishes or test
tubes. Cool tubes in a slanted position.
Use:  For differentiation between group D streptococci and nongroup
D streptococci. To differentiate members of the Enterobacteriaceae,
particularly Klebsiella, Enterobacter, and Serratia, from other enteric
bacteria. To differentiate Listeria monocytogenes. Bile tolerance and
esculin hydrolysis (seen as a dark brown to black complex) are pre-
sumptive for enterococci (group D streptococci).