Bile Esculin Agar (BAM M18)

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Esculin Agar
(BAM M18)

培养基配方
Composition per liter:
Oxgall ......................................................40.0g
Agar.........................................................15.0g
Pancreatic digest of gelatin..................................5.0g
Beef extract..................................................3.0g
Esculin ......................................................1.0g
Ferric citrate................................................0.5g
pH 6.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Distribute into sterile Petri dishes or test tubes. Cool tubes in a slanted
position.
Use:  For differentiation between group D streptococci and nongroup
D streptococci. To differentiate members of the Enterobacteriaceae,
particularly Klebsiella, Enterobacter, and Serratia, from other enteric
bacteria. To differentiate Listeria monocytogenes. Bile tolerance and
esculin hydrolysis (seen as a dark brown to black complex) are pre-
sumptive for enterococci (group D streptococci).