BG Sulfa HiVeg Agar (Brilliant Green Sulfa HiVeg Agar)

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG Sulfa HiVeg Agar
(Brilliant Green Sulfa HiVeg Agar)

培养基配方
Composition per liter:
Agar.......................................................20.0g
Plant peptone No.3.........................................10.0g
Lactose....................................................10.0g
Sucrose....................................................10.0g
NaCl........................................................5.0g
Yeast extract...............................................3.0g
Sodium sulphapyridine.......................................1.0g
Phenol Red.................................................0.08g
Brilliant Green...........................................12.5mg
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently to boil-
ing. Distribute into tubes or flasks. Autoclave for no longer than 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes if desired.
Use:  For the selective isolation of Salmonella species other than Sal-
monella typhi from food, dairy products, eggs and egg products, and
feed. Salmonella appear as red, pink, or white colonies surrounded by
zones of bright red.