BG Sulfa Agar (Brilliant Green Sulfapyridine Agar)

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG Sulfa Agar
(Brilliant Green Sulfapyridine Agar)

培养基配方
Composition per liter:
Agar ........................................................20.0g
Proteose peptone No.3........................................10.0g
Lactose......................................................10.0g
Sucrose......................................................10.0g
NaCl..........................................................5.0g
Yeast extract.................................................3.0g
Sodium sulfapyridine..........................................1.0g
Brilliant Green ............................................0.125g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems .
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently to boil-
ing. Distribute into tubes or flasks. Autoclave for no longer than 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes if desired.
Use:  For the selective isolation of Salmonella species other than Sal-
monella typhi from food, dairy products, eggs and egg products, and
feed. Salmonella appear as red, pink, or white colonies surrounded by
zones of bright red.