Betabacterium Medium

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Betabacterium Medium

培养基配方
Composition per liter:
Pancreatic digest of casein..................................10.0g
Agar ........................................................10.0g
Yeast extract.................................................5.0g
Glucose ......................................................5.0g
K2HPO4 .......................................................2.0g
Liver extract..............................................100.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add 1 pound of finely ground beef liver
to 2.0L of distilled/deionized water. Autoclave for 2.5–3 hr at 15 psi
pressure–121°C under flowing steam. The liquid should become fluo-
rescent yellow. Filter through sterile cheesecloth. Save solids and dry
at 50°C. Add a few pieces of the dried liver to sterile test tubes or
flasks. Prepare basal medium by adding components to distilled/deion-
ized water and bring volume to 1.0L. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add sterile basal medium to each test tube
or flask containing liver. Commercial liver extract may be used at a
concentration of 0.1%.
Use:  For the growth and maintenance of Lactobacillus species. Beta-
bacterium is an archaic name that was used to describe several bacteria
as a subgenus of the Lactobacillus group.