BCP D Agar (Bromcresol Purple Deoxycholate Agar)

[所属分类:培养基配方] [发布时间:2019-12-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCP D Agar
(Bromcresol Purple Deoxycholate Agar)

培养基配方
Composition per liter:
Agar ...........................................................25.0g
Lactose.........................................................10.0g
Sucrose.........................................................10.0g
Pancreatic digest of casein......................................7.5g
Thiopeptone......................................................7.5g

NaCl.............................................................5.0g
Yeast extract....................................................2.0g
Sodium citrate...................................................2.0g
Sodium deoxycholate..............................................1.0g
Bromcresol Purple ..............................................0.02g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Pour into sterile Petri dishes without sterilization. Do not au-
toclave. Use the same day.
Use: For the isolation, cultivation, and differentiation of Gram-negative
enteric bacilli from clinical and nonclinical specimens. For the isolation,
cultivation, and identification of microorganisms from fecal specimens.
For the isolation and cultivation of Salmonella, Shigella, and other nonlac-
tose- and nonsucrose-fermenting microorganisms. Nonlactose/nonsucrose
fermenting microorganisms appear as colorless or blue colonies. Lactose/
sucrose-fermenting microorganisms, such as coliform bacteria, appear as
yellow-opaque white colonies surrounded by a zone of precipitated deoxy-
cholate.