BCP Azide Broth (Bromcresol Purple Azide Broth)

[所属分类:培养基配方] [发布时间:2019-12-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCP Azide Broth
(Bromcresol Purple Azide Broth)

培养基配方
Composition per liter:
Casein peptone..............................................10.0g
Yeast extract...............................................10.0g
D- Glucose...................................................5.0g
NaCl.........................................................5.0g
K2HPO4 ......................................................2.7g
KH2PO4 ......................................................2.7g
NaN3 ........................................................0.5g
Bromcresol Purple..........................................0.032g
pH 6.9 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water to 1.0L. Mix thoroughly. Gently heat to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For use in the confirmation test for the presence of fecal strepto-
cocci in water and wastewater.