BCP DCLS Agar (Bromcresol Purple Deoxycholate Citrate Lactose Sucrose Agar)

[所属分类:培养基配方] [发布时间:2019-12-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCP DCLS Agar
(Bromcresol Purple Deoxycholate
Citrate Lactose Sucrose Agar)

培养基配方
Composition per liter:
Agar ........................................................14.0g
Sodium citrate...............................................10.0g
Lactose.......................................................7.5g
Sucrose.......................................................7.5g
Pancreatic digest of casein...................................7.5g
Peptone.......................................................7.5g
NaCl..........................................................5.0g
Na2S2O3 ·5H2O................................................5.0g
Yeast extract.................................................3.0g
Meat extract .................................................3.0g
Sodium deoxycholate...........................................2.5g
Bromcresol Purple ...........................................0.02g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Pour into sterile Petri dishes without sterilization. Do not au-
toclave. Use the same day.
Use: For the differential isolation of Gram-negative enteric bacilli
from clinical and nonclinical specimens. For the isolation and identifi-
cation of microorganisms from fecal specimens. For the isolation of
Salmonella, Shigella, and other nonlactose- and nonsucrose-ferment-
ing microorganisms. Nonlactose/nonsucrose-fermenting microorgan-
isms appear as colorless or blue colonies. Lactose/sucrose-fermenting
microorganisms, such as coliform bacteria, appear as yellow-opaque
white colonies surrounded by a zone of precipitated deoxycholate.