BAM SM Agar (ATCC Medium 1656)

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BAM SM Agar
(ATCC Medium 1656)

培养基配方
Composition per liter:
Agar.......................................................20.0g
Yeast extract...............................................6.0g
Glucose.....................................................5.0g
KH2PO4 .....................................................3.0g
MgSO4 ·7H2O................................................0.5g
CaCl2 ·2H2O ..............................................0.25g
(NH4)2SO4 ..................................................0.2g
Trace elements.............................................1.0mL
pH 4.0 ± 0.2 at 25°C
Trace Elements:
Composition per liter:
CaCl2 ·2H2O ..............................................0.66g
Na2MoO4 ·2H2O..............................................0.3g
ZnSO4 ·7H2O...............................................0.18g
CoCl2 ·6H2O...............................................0.18g
CuSO4 ·5H2O...............................................0.16g
MnSO4 ·4H2O...............................................0.15g
H3BO3 ......................................................0.1g
Preparation of Trace Elements: Add components to 1.0L of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 800.0mL. Mix thoroughly.
Gently heat and bring to boiling. Adjust medium to pH 4.0 with H2SO4 .
Add agar to 200.0mL of distilled/deionized water. Autoclave agar sep-
arately to avoid acid hydrolysis. Autoclave for 15 min at 15 psi pres-
sure–121°C. Mix the two solutions together. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Bacillus cycloheptanicus.