BAM Broth

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BAM Broth

培养基配方
Composition per liter:
Glucose .......................................................5.0g
KH2PO4 ........................................................3.0g
Yeast extract..................................................1.0g
MgSO4 ·7H2O...................................................0.5g
CaCl2 ·2H2O..................................................0.25g
(NH4)2SO4 .....................................................0.2g
Trace elements................................................1.0mL
pH 4.0 ± 0.2 at 25°C
Trace Elements:
Composition per liter:
CaCl2 ·2H2O...................................................0.66g
Na2MoO4 ·2H2O .................................................0.3g
ZnSO4 ·7H2O...................................................0.18g
CoCl2 ·6H2O ..................................................0.18g
CuSO4 ·5H2O...................................................0.16g
MnSO4 ·4H2O...................................................0.15g
H3BO3 ..........................................................0.1g
Preparation of Trace Elements:  Add components to 1.0L of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust medium to pH 4.0 with H2SO4 . Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Bacillus acidoterrestris.