Baird-Parker Medium (BAM M17)

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baird-Parker Medium
(BAM M17)

培养基配方
Composition per liter:
Agar ..........................................................20.0g
Glycine........................................................12.0g
Sodium pyruvate................................................10.0g
Pancreatic digest of casein....................................10.0g
Beef extract....................................................5.0g
LiCl·6H2O......................................................5.0g
Yeast extract...................................................1.0g
Egg yolk tellurite enrichment ................................50.0mL
pH 7.0 ± 0.2 at 25°C
Egg Yolk Tellurite Enrichment:
Composition per 100.0mL :
Chicken egg yolks................................................10
K2TeO3 .......................................................0.15g
NaCl (0.9% solution).........................................50.0mL
Preparation of Egg Yolk Tellurite Enrichment:  Soak eggs with
1:100 dilution of saturated mercuric chloride solution for 1 min. Crack
11 eggs and separate yolks from whites. Mix egg yolks. Measure
30.0mL of egg yolk emulsion and add to 70.0mL of 0.9% NaCl solu-
tion. Mix thoroughly. Add 0.15g K2 TeO3 . Filter sterilize. Warm to 45°–
50°C.
Caution: Potassium tellurite is toxic.
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except egg yolk tellu-
rite enrichment, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 48°–50°C. Aseptically
add 50.0mL of sterile egg yolk tellurite enrichment. Mix thoroughly.
Pour into sterile Petri dishes. The medium must be densely opqaue.
Dry plates before use. Plates can be stored for up to 5 days at 20–25°C
before use.
Use: For the selective isolation and enumeration of coagulase-positive
staphylococci from foods.