Balamuth Medium

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Balamuth Medium

培养基配方
Composition per 200.0mL:
Dehydrated egg yolk..........................................36.0g
Dried liver concentrate ......................................1.0g
Rice starch ..................................................0.2g
Potassium phosphate buffer, pH 7.5.........................125.0mL
NaCl solution..............................................125.0mL
pH 7.3 ± 0.2 at 25°C
NaCl Solution
Composition per 200.0mL:
NaCl..........................................................1.6g
Preparation of NaCl Solution: Add NaCl to distilled/deionized
water and bring volume to 200.0mL. Mix thoroughly.
Potassium Phosphate Buffer, 0.067M
Composition per 200.0mL:
K2HPO4 (1M solution).........................................8.6mL
KH2PO4 (1M solution)........................................4.66mL
Preparation of Potassium Phosphate Buffer: Combine the
K2HPO4 and KH2PO4 solutions. Bring volume to 200.0mL with dis-
tilled/deionized water. Adjust pH to 7.5.
Preparation of Medium: Add dehydrated egg yolk to 36.0mL of
distilled/deionized water. Add 125.0mL of 0.8% NaCl. Mix thoroughly
in a blender. Heat in a covered, double boiler until infusion reaches
80°C and maintain at this temperature for 20 min. Add 20.0mL of dis-
tilled/deionized H 2 O. Filter through a layer of cheesecloth. To 90–
100.0mL of filtrate add 0.8% NaCl solution to bring volume to
125.0mL. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 4°C.
Filter. To filtrate, add an equal volume of 0.067M potassium phosphate
buffer, pH 7.5. Add 1.0g of dried liver concentrate. Mix thoroughly.
Distribute into tubes or flasks in 10.0mL volumes. Autoclave for 20 min
at 15 psi pressure–121°C. Prior to inoculation, add 0.01g of rice starch to
each tube.
Use: For the cultivation and maintenance of Entamoeba histolytica
and other intestinal protozoa.